icam 5 monoclonal antibodies  (R&D Systems)

Journal: eLife

Article Title: Functionally heterogeneous human satellite cells identified by single cell RNA sequencing

doi: 10.7554/eLife.51576

Figure Lengend Snippet: ( a ) Feature plots displaying localized gene expression for the genes DLK1, ICAM1, and VCAM1 within the 2D UMAP space of the eight combined vasti, the rectus femoris, the two combined rectus abdominis and the two combined pectoralis majors as shown in and , . Each dot represents a single cell. Deeper purple coloration represents increased expression. ( b ) Immunofluorescence staining of human satellite cells for validation markers within sections of human muscle. Pink arrows denote human satellite cells that are positive for the marker (scale, 50 µm). White arrowheads mark satellite cells that are negative for expression of the marker. (n = 3, biological replicates). ( c ) Bar plot displaying the quantification of DLK1, ICAM1, and VCAM1 expression of human satellite cells with both immunofluorescence staining and flow cytometry. (n = 3, biological replicates). Data presented as mean ± SEM. Figure 2—source data 1. Validation of satellite cell clusters.

Article Snippet: Antibody , Mouse monoclonal anti-ICAM1 (G-5) , Santa Cruz Biotechnology , sc-8439 , IF(1:50).

Techniques: Expressing, Immunofluorescence, Staining, Marker, Flow Cytometry

Journal: eLife

Article Title: Functionally heterogeneous human satellite cells identified by single cell RNA sequencing

doi: 10.7554/eLife.51576

Figure Lengend Snippet: ( a-c ) Representative flow cytometry profiles for CXCR4+/CD29+/CD56+ human satellite cells expressing ( a ) ICAM1, ( b ) VCAM1, and ( c ) DLK1. (n = 3, biological replicates). ( d ) Representative immunofluorescence co-staining of PAX7+ human satellite cells for DLK1/ICAM, VCAM1/ICAM1 and VCAM1/DLK1 (scale, 50 µm). Lavender arrows mark double positive PAX7+ cells, white arrowheads denote single positive PAX7+ cells. (n = 3, biological replicates). ( e ) Bar graph showing the proportion of double and single positive satellite cells for DLK1, VCAM1 and ICAM1 in human muscle as quantified by immunofluorescence staining (n = 3, biological replicates). *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001. Data presented as mean ± SEM.

Article Snippet: Antibody , Mouse monoclonal anti-ICAM1 (G-5) , Santa Cruz Biotechnology , sc-8439 , IF(1:50).

Techniques: Flow Cytometry, Expressing, Immunofluorescence, Staining

Journal: eLife

Article Title: Functionally heterogeneous human satellite cells identified by single cell RNA sequencing

doi: 10.7554/eLife.51576

Figure Lengend Snippet:

Article Snippet: Antibody , Mouse monoclonal anti-ICAM1 (G-5) , Santa Cruz Biotechnology , sc-8439 , IF(1:50).

Techniques: Plasmid Preparation, Recombinant, Blocking Assay, Software